X-gal Staining of Drosophila Embryos Compatible with Antibody Staining or In Situ Hybridization
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چکیده
منابع مشابه
X-gal staining of Drosophila embryos compatible with antibody staining or in situ hybridization.
the slow development. The low background especially applies for detection of digoxigenin-labeled RNA probes. When detecting identical probes labeled with fluorescein, the background is higher, presumably because of nonspecific binding of the fluorescein antibody (the higher background is detected with both BCIP and NBT/BCIP). In cases in which the investigated signals are of different intensity...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98246bm03